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Updated: May 9, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Published on: July 28, 2016

Measuring myeloperoxidase activity in biological samples.

Benjamin Pulli¹, Muhammad Ali, Reza Forghani

¹Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

|July 18, 2013

Summary

A new, validated assay protocol provides specific and sensitive measurement of myeloperoxidase (MPO) activity in biological samples. This method distinguishes between extracellular MPO and intracellular MPO, a marker for neutrophil infiltration.

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Area of Science:

Biochemistry
Immunology
Pathology

Background:

Myeloperoxidase (MPO) is an enzyme implicated in various diseases.
Current MPO activity assays lack specificity, sensitivity, and uniformity.
Standardization is crucial for reliable MPO activity measurements in biological samples.

Purpose of the Study:

To establish and validate a robust MPO activity assay protocol.
To differentiate between extracellular and intracellular MPO activity.
To correlate MPO activity with disease markers and neutrophil content.

Main Methods:

Developed a protocol to isolate extra- and intracellular MPO from murine organs.
Employed antibody capture of MPO followed by ADHP assessment.
Validated the assay using MPO-knockout mice and disease models (multiple sclerosis, steatohepatitis, myocardial infarction).

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Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Published on: May 12, 2023

Related Experiment Videos

Last Updated: May 9, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Published on: July 28, 2016

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Published on: May 12, 2023

Main Results:

The validated protocol demonstrated high specificity and reproducibility for MPO activity assays.
The ADHP method showed high sensitivity across a broad range.
Intracellular MPO activity correlated significantly with tissue neutrophil content, serving as a marker for neutrophil infiltration.

Conclusions:

A highly specific and sensitive MPO activity assay protocol has been validated for use in biological samples.
The protocol allows for the assessment of both extracellular MPO activity (oxidative stress) and intracellular MPO activity (neutrophil infiltration).
This standardized method is recommended for all future MPO activity assays.