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Updated: May 9, 2026
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Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
Published on: October 13, 2017
[Tear fluid proteome variability in healthy donors].
Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova
|July 19, 2013
Summary
This study analyzed individual healthy donor tear proteomes, revealing significant variability in minor proteins but consistent levels of 6 major proteins. Lacritin glycosylation was a common finding in healthy tear fluid.
Area of Science:
- Clinical proteomics
- Biochemistry
- Analytical chemistry
Context:
- Variability in control samples is a major challenge in clinical proteomics.
- Existing studies often use pooled samples, limiting the assessment of normal protein content variability.
- Reliable sources for estimating protein variability in normal human tear proteomes are scarce.
Purpose:
- To estimate the variability of protein content in healthy donor tear fluid.
- To analyze individual protein profiles using high-performance liquid chromatography and tandem mass spectrometry.
- To investigate the presence and typicality of Lacritin glycosylation in healthy tear fluid.
Summary:
- Analyzed protein profiles from 11 individual healthy donors using HPLC and MS/MS.
- Revealed significant variability in minor tear proteins, while 6 major proteins remained invariable across all samples.
- Identified Lacritin glycosylation in all healthy donor tear samples, suggesting it's a typical modification.
Impact:
- Establishes a method for assessing protein variability in individual tear samples.
- Highlights the importance of considering individual variability in clinical proteomics studies.
- Provides foundational data for identifying biomarkers for ophthalmologic diseases by analyzing pool samples.
