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Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
Variability in oxidative stress biomarkers following a maximal exercise test
Anna L Mullins1, Simon P van Rosendal, David R Briskey
1Exercise and Oxidative Stress Research Group, School of Human Movement Studies, the University of Queensland, Brisbane, Queensland, Australia.
Summary
Maximal exercise significantly increases oxidative stress markers like F2-isoprostanes and protein carbonyls. However, the response shows substantial between-individual variability, indicating potential for personalized redox homeostasis assessment.
Area of Science:
- Exercise physiology
- Biochemistry
- Clinical biomarkers
Background:
- Maximal exercise induces oxidative stress, a key factor in cellular damage.
- Assessing redox homeostasis through biomarkers is crucial for clinical applications.
- Understanding variability in oxidative stress response is essential for interpreting results.
Purpose of the Study:
- To quantify the between-individual variability in exercise-induced changes in oxidative stress markers.
- To identify potential predictors of these variable responses to maximal exercise.
- To explore the utility of oxidative stress markers as clinical biomarkers for redox homeostasis.
Main Methods:
- Measurement of plasma F2-isoprostanes (Isop), protein carbonyls (PCs), glutathione peroxidase (GPX) activity, and total antioxidant capacity (TAC).
- Samples collected before and after a maximal treadmill exercise test.
- Analysis of exercise-induced changes and between-individual coefficients of variation.
Main Results:
- Maximal exercise significantly increased Isop (27.0%), PC (6.2%), and GPX (7.8%).
- Large between-individual variability was observed: Isop (152%), PC (240%), GPX (130%), and TAC (243%).
- Significant increases in oxidative stress markers were noted post-exercise.
Conclusions:
- Maximal exercise demonstrably impacts oxidative stress markers.
- There is substantial inter-individual variability in the oxidative stress response to exercise.
- These findings highlight the potential of exercise-induced oxidative stress markers for assessing redox homeostasis, but variability must be considered.
