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Updated: May 9, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
Generation and characterization of a new solid form of trehalose
Sarah J Pyszczynski1, Eric J Munson
1Department of Pharmaceutical Sciences, University of Kentucky , Lexington, Kentucky 40536, United States.
Abstract:
We have identified and characterized a new solid form of trehalose, the δ form (Tδ) using (13)C solid-state NMR spectroscopy (SSNMR). Tδ is formed from dehydrations of trehalose dihydrate (T(h)) performed at or below 100 °C, and it is generated concurrently with the α form of trehalose (Tα) and amorphous trehalose (T(am)); the β form of trehalose (Tβ) has been observed in some dehydrated samples. Differential scanning calorimetry (DSC) and powder X-ray diffraction (PXRD) studies of samples containing Tδ do not indicate the presence of the new form. Tδ is metastable: upon exposure to temperatures above 80 °C, it converts to either Tα or Tam, and upon exposure to ambient humidity, it reverts to T(h).
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