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Related Concept Videos

Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

833
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
833
NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

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The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
1.7K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

934
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
934
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.3K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.3K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.0K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.0K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.1K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.1K

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Updated: Sep 20, 2025

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
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Quantitative Solid-State NMR Spectroscopy (qSSNMR) in Pharmaceutical Analysis.

Zhaoxi Zheng1, Kang Chen2, Yang Liu3

  • 1Department of Chemistry, Brandeis University, Waltham, Massachusetts, USA.

Magnetic Resonance in Chemistry : MRC
|May 26, 2025
PubMed
Summary
This summary is machine-generated.

Quantitative solid-state NMR (qSSNMR) enhances pharmaceutical analysis for drug development. Advancements improve precision, speed, and analysis of complex mixtures, addressing adoption challenges.

Keywords:
complex drug formulationspharmaceutical analysisqSSNMRquantitative solid‐state NMRsolid‐state characterization

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Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Sciences
  • Materials Science

Background:

  • Pharmaceutical analysis is critical for drug development and quality control.
  • Ensuring drug safety and efficacy relies on precise analytical methods.
  • Quantitative solid-state NMR (qSSNMR) is a vital technique for solid drug formulations.

Purpose of the Study:

  • To review the evolution and advancements in quantitative solid-state NMR (qSSNMR).
  • To highlight technical improvements in qSSNMR for pharmaceutical applications.
  • To discuss the application of qSSNMR in analyzing complex pharmaceutical mixtures.

Main Methods:

  • Review of literature on quantitative solid-state NMR (qSSNMR) techniques.
  • Analysis of improvements in detection limits, resolution, and throughput.
  • Exploration of qSSNMR applications in pharmaceutical analysis.

Main Results:

  • Significant improvements in qSSNMR detection limits and resolution.
  • Enhanced high-throughput capabilities for faster analysis.
  • Successful application of qSSNMR in analyzing complex pharmaceutical mixtures.

Conclusions:

  • qSSNMR technology has evolved significantly, offering enhanced capabilities for pharmaceutical analysis.
  • Ongoing efforts in automation and user-friendly software aim to increase qSSNMR adoption.
  • qSSNMR is a powerful tool for drug development and quality assurance.