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Updated: Jul 31, 2026

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Chromatographic Purification of Highly Active Yeast Ribosomes
Published on: October 24, 2011
Purification of pyruvate kinase from human erythrocytes by immunoaffinity chromatography
1Institute of Biochemistry, Humboldt University, Berlin, GDR.
Abstract:
An one-step-purification of native pyruvate kinase from human erythrocytes by immunoaffinity chromatography with monoclonal antibodies coupled on CNBr-activated Sepharose 4B is described. The recoveries of PK amounted up to 83%, with a 2400 fold purification of PK-R from human stroma free hemolysate.

