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Adrenergic Receptors: β Subtype01:26

Adrenergic Receptors: β Subtype

β-adrenoceptors have varied sensitivities towards adrenaline, noradrenaline, and isoprenaline. The order of agonist potency is as follows:
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Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
Adrenergic Receptors: ɑ Subtype01:31

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Adrenergic Receptors (Adrenoceptors): Classification

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α-Adrenoceptors
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RNA Editing02:23

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HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
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Published on: July 20, 2016

Adenosine receptor expression and gene reference evaluation in human leukocytes.

Laura Sabatino1, Manuela Cabiati, Chiara Caselli

  • 1CNR Institute of Clinical Physiology, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.

Clinical Laboratory
|July 20, 2013
PubMed
Summary

This study developed a rapid RNA extraction method from leukocytes to analyze adenosine receptor (AR) mRNA expression in whole blood. Stable reference genes were identified, revealing distinct expression patterns for different AR subtypes in normal subjects.

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Area of Science:

  • Molecular Biology
  • Immunology
  • Pharmacology

Background:

  • Challenges exist in using peripheral blood mononuclear cells and polymorphonuclear neutrophils for gene expression studies.
  • A need for a simplified and efficient method for RNA extraction from leukocytes in human whole blood (WB) is evident.
  • Understanding adenosine receptor (AR) mRNA expression and identifying stable reference genes are crucial for accurate gene expression analysis.

Purpose of the Study:

  • To establish a fast and easy total RNA extraction procedure from leukocytes in < 10 mL of human whole blood (WB).
  • To quantify adenosine receptor (AR) mRNA expression levels in WB samples from healthy individuals.
  • To identify the most stable reference genes for normalizing AR mRNA expression data.

Main Methods:

  • Total RNA was extracted from leukocytes isolated from human whole blood.
  • Messenger RNA (mRNA) expression of adenosine receptors (ARs) was quantified using Real-Time Polymerase Chain Reaction (RT-PCR).
  • Gene expression data were normalized using the most stable reference genes identified in the study.

Main Results:

  • TPT1, EEF1A, and RPL13A were identified as the most stably expressed reference genes.
  • Similar mRNA expression levels were observed for adenosine receptor subtypes A2aR, A2bR, and A3R.
  • Adenosine receptor A1R (A1R) showed significantly lower mRNA expression compared to A2aR (p = 0.02) and A3R (p = 0.04).

Conclusions:

  • The developed RNA extraction method provides a valuable tool for leukocyte gene expression analysis.
  • This study establishes a baseline for adenosine receptor (AR) mRNA expression in normal human whole blood.
  • The findings serve as a foundation for future research into AR expression in various human diseases.