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Updated: May 9, 2026

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
Published on: October 17, 2014
Atypical cadherin negotiates a turn.
Dongbo Shi1, Toshihiko Fujimori, Tadashi Uemura
1Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
Planar cell polarity (PCP) signaling orients lymphatic valve formation. The atypical cadherin Celsr1 localizes to cell protrusions, guiding valve-forming cells perpendicular to the lymphatic vessel axis.
Area of Science:
- Cell biology
- Developmental biology
- Molecular biology
Background:
- Planar cell polarity (PCP) signaling regulates cell polarization and tissue development.
- Lymphatic vascular development involves precise cell behaviors for valve formation.
Purpose of the Study:
- To investigate the role of PCP signaling in lymphatic valve formation.
- To elucidate the function of the atypical cadherin Celsr1 in this process.
Main Methods:
- Immunofluorescence microscopy to visualize Celsr1 localization.
- Analysis of lymphatic endothelial cell behavior in developing lymphatic vessels.
Main Results:
- Celsr1 shows transient localization to cellular protrusions in lymphatic endothelial cells.
- This localization is crucial for orienting valve-forming cells perpendicular to the vessel axis.
Conclusions:
- Celsr1 plays a key role in establishing cell polarity during lymphatic valve development.
- The study reveals a novel mechanism for orienting cells in lymphatic endothelial tissues.
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