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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Related Experiment Video

Updated: May 9, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

Localization-based super-resolution imaging of cellular structures.

Pakorn Kanchanawong¹, Clare M Waterman

¹Department of Bioengineering, Mechanobiology Institute, National University of Singapore, Singapore, Singapore.

Methods in Molecular Biology (Clifton, N.J.)

|July 23, 2013

Summary

This summary is machine-generated.

Super-resolution microscopy breaks the diffraction limit for cellular imaging, achieving ~20 nm resolution. This study details protocols for single molecule localization microscopy, enabling visualization of protein structures within cells.

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Last Updated: May 9, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

Super-resolution Imaging of the Bacterial Division Machinery

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Cellular and Molecular Biology
Biophysics
Microscopy

Background:

Conventional fluorescence microscopy is limited by diffraction (~250 nm resolution).
Super-resolution microscopy techniques overcome these limitations, achieving nanoscale resolution (~20 nm).

Purpose of the Study:

To describe protocols for super-resolution imaging using single molecule localization microscopy (SMLM).
To demonstrate the application of SMLM for visualizing focal adhesion proteins.

Main Methods:

Single molecule localization microscopy (SMLM) based on photoswitchable fluorophores.
SMLM using photoactivatable fluorescent proteins.
Imaging of fluorescently tagged focal adhesion proteins.

Main Results:

Developed and validated SMLM protocols for high-resolution imaging of cellular structures.
Achieved resolution approaching the scale of single proteins (~20 nm).

Conclusions:

The described SMLM protocols enable visualization of macromolecular assemblies at the nanoscale.
These methods are adaptable for diverse biological imaging applications involving fluorescently tagged components.