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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...

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Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
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Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

Quantitative electron tomography: the effect of the three-dimensional point spread function.

Hamed Heidari1, Wouter Van den Broek, Sara Bals

  • 1EMAT, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.

Ultramicroscopy
|July 23, 2013
PubMed
Summary
This summary is machine-generated.

Electron tomography reconstructions contain artifacts affecting quantitative analysis. This study corrects intensity variations using the point spread function (PSF) to improve particle size estimation in high-angle annular dark-field scanning transmission electron microscopy.

Keywords:
Artifact correctionElectron tomographyRadius distribution

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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

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Last Updated: May 9, 2026

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
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Published on: July 5, 2016

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

Area of Science:

  • Electron microscopy
  • Materials science
  • Image processing

Background:

  • Three-dimensional (3D) reconstructions from electron tomography are susceptible to experimental imperfections.
  • These artifacts, such as variations in intensity, hinder accurate quantitative interpretation of tomographic data.

Purpose of the Study:

  • To correct artificial intensity variations in 3D tomographic reconstructions.
  • To improve the quantitative accuracy of electron tomography by addressing artifacts.
  • To refine particle radius estimation in high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM).

Main Methods:

  • Determining the 3D point spread function (PSF) of a tomographic reconstruction.
  • Utilizing high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) data.
  • Quantifying and correcting for errors introduced by the PSF a posteriori.

Main Results:

  • The study quantifies the underestimation of smaller particle intensities caused by the large tails of the PSF.
  • A method for correcting these PSF-induced errors is presented.
  • The correction improves the accuracy of radius estimates for smaller particles.

Conclusions:

  • Correction for the point spread function (PSF) is crucial for accurate quantitative analysis in electron tomography.
  • The developed method effectively addresses intensity variations and improves particle size determination.
  • This approach enhances the reliability of quantitative data obtained from HAADF-STEM tomographic reconstructions.