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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: May 9, 2026

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Perspectives for using genetically encoded fluorescent biosensors in plants.

Sisse K Gjetting1, Alexander Schulz, Anja T Fuglsang

  • 1Transport Biology Section, Department of Plant and Environmental Sciences, University of Copenhagen Copenhagen, Denmark.

Frontiers in Plant Science
|July 23, 2013
PubMed
Summary

Genetically encoded fluorescent biosensors offer powerful live imaging in plants. This review highlights successful experiments and challenges, paving the way for broader plant biosensor applications.

Keywords:
calcium sensorfluorescence microscopygenetically encoded biosensorspH sensorsphosphatidylinositol sensorplant bioimaging

Related Experiment Videos

Last Updated: May 9, 2026

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Area of Science:

  • Plant biology
  • Cell imaging
  • Biotechnology

Background:

  • Genetically encoded fluorescent biosensors are vital for quantitative live imaging.
  • Plant applications lag behind mammalian systems in sensor variety and tissue types.
  • Bridging this gap is crucial for advancing plant science.

Purpose of the Study:

  • To review successful physiological experiments using fluorescent biosensors in plant tissues.
  • To identify challenges faced by plant cell biologists in biosensor application.
  • To discuss strategies for overcoming these challenges and future prospects.

Main Methods:

  • Literature review of published studies.
  • Compilation of a table detailing successful plant biosensor experiments.
  • Analysis of experimental challenges and solutions.

Main Results:

  • Documented successful applications of fluorescent biosensors in various plant physiological studies.
  • Identified key technical and biological hurdles in plant biosensor implementation.
  • Highlighted innovative approaches to overcome limitations.

Conclusions:

  • Fluorescent biosensors are powerful tools for plant research, despite existing challenges.
  • Continued development and adaptation of biosensors will expand their utility in plants.
  • Future research should focus on sensor diversity and broader tissue-specific applications.