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Related Concept Videos

Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...

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Related Experiment Video

Updated: May 9, 2026

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

Stem cell maintenance in a different niche.

Jeong Mook Lim1, Ji Yeon Ahn, Seung Tae Lee

  • 1WCU Biomodulation Program, Seoul National University, Seoul, Korea. ; Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.

Clinical and Experimental Reproductive Medicine
|July 23, 2013
PubMed
Summary
This summary is machine-generated.

Controlling stem cell behavior is key for regenerative medicine. This study reviews a novel 3D non-cellular niche that optimizes the microenvironment to support stem cell self-renewal and function.

Keywords:
Extracellular matrixNon-cellular nicheSelf-renewalStem cellsThree-dimensional

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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
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Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Controlling stem cell fate and function is crucial for regenerative medicine applications.
  • Non-cellular niches modulating the biophysical microenvironment can support stem cell self-renewal.
  • Previous studies highlighted the impact of extracellular matrix microgeometry on stem cell behavior.

Purpose of the Study:

  • To review a designed three-dimensional, non-cellular niche for supporting stem cell self-renewal.
  • To discuss the characteristics of stem cells within this designed system.

Main Methods:

  • Review of studies focusing on non-cellular niche design.
  • Analysis of biophysical and chemical factors influencing stem cell behavior.
  • Examination of microgeometry's role in stem cell phenotype.

Main Results:

  • A three-dimensional, non-cellular niche can be designed to support stem cell self-renewal.
  • Optimized biophysical factors and microgeometry significantly influence stem cell behavior.
  • The designed niche modulates the biophysical microenvironment effectively.

Conclusions:

  • The reviewed 3D non-cellular niche shows promise for controlling stem cell self-renewal.
  • Further discussion on stem cell characteristics within this system is warranted.
  • This approach offers a potential strategy for advancing regenerative medicine therapies.