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Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

Argon cluster ion source evaluation on lipid standards and rat brain tissue samples.

Claudia Bich1, Rasmus Havelund, Rudolf Moellers

  • 1Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France.

Analytical Chemistry
|July 24, 2013
PubMed
Summary

New argon cluster ion sources enable sensitive, damage-free depth profiling of organic materials and biological tissues. This technique enhances imaging sensitivity for time-of-flight secondary ion mass spectrometry (TOF-SIMS) in biological samples.

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biophysics

Background:

  • Argon cluster ion sources provide projectiles for sputtering and secondary ion mass spectrometry.
  • These projectiles deposit energy in the top nanometers of organic materials, leading to high sputtering yields and minimal surface damage.

Purpose of the Study:

  • To evaluate the feasibility of dual beam depth profiling experiments in organic materials using argon cluster ion sources.
  • To assess the direct application of this technique on biological tissue samples for enhanced imaging sensitivity.

Main Methods:

  • Utilized argon cluster ion beams (hundreds of atoms, 10-20 keV) for sputtering and depth profiling.
  • Applied time-of-flight secondary ion mass spectrometry (TOF-SIMS) for biological imaging of rat brain tissue sections (14 μm thick).

Main Results:

  • Demonstrated successful dual beam depth profiling on model organic samples.
  • Achieved enhanced sensitivity in TOF-SIMS biological imaging of rat brain tissue.
  • Observed variations in chemical composition with depth, notably for cholesterol.
  • Identified potential matrix effects related to cholesterol presence or absence.

Conclusions:

  • Argon cluster ion sources facilitate damage-free depth profiling in organic materials.
  • The technique significantly enhances the sensitivity of TOF-SIMS for biological imaging.
  • Depth-dependent chemical variations and matrix effects warrant further investigation in complex biological samples.