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Enhancing adherence of Arcobacter butzleri after serial intraperitoneal passages in mice
Heriberto Fernández1, Sandra Flores, María P Villanueva
1Instituto de Microbiología Clínica, Universidad Austral de Chile, Valdivia, Chile. hfernand@uach.cl
Abstract:
We investigated the possibility of enhancing the adherence capacity of four low-adherent Arcobacter butzleri strains after serial intraperitoneal passage (i.p.) in mice. All the strains enhanced their adherence capacity after the first passage, increasing their adhesion rates after each passage. These results suggest that i.p. passage enhances the expression of adherence in A. butzleri strains.
Insights
Serial passage in mice significantly boosted the adherence of low-adherent Arcobacter butzleri strains. This suggests intraperitoneal passage enhances bacterial adhesion capabilities.
Area of Science:
- Microbiology
- Bacterial Pathogenesis
- Infectious Diseases
Background:
- Arcobacter butzleri is a foodborne pathogen known for its low adherence.
- Understanding bacterial adherence is crucial for developing effective treatments and prevention strategies.
Purpose of the Study:
- To investigate methods for enhancing the adherence capacity of low-adherent Arcobacter butzleri strains.
- To determine if serial intraperitoneal passage in mice influences bacterial adherence.
Main Methods:
- Four low-adherent Arcobacter butzleri strains were subjected to serial intraperitoneal passage in mice.
- Adherence capacity was assessed after each passage to quantify changes.
Main Results:
- All tested Arcobacter butzleri strains demonstrated enhanced adherence capacity following the first intraperitoneal passage.
- A consistent increase in adhesion rates was observed with each subsequent passage.
- The study confirmed a dose-dependent enhancement of adherence with repeated passage.
Conclusions:
- Serial intraperitoneal passage in mice is an effective method for enhancing the adherence capacity of Arcobacter butzleri.
- Intraperitoneal passage appears to upregulate the expression of adherence factors in Arcobacter butzleri strains.
- These findings have implications for understanding bacterial adaptation and potential virulence in vivo.
