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Isolation of lysozyme on chitosan.
Biotechnology and Bioengineering
|January 1, 1978
Summary
Lysozyme was successfully immobilized onto chitosan without reagents. Optimal pH and a 2% propylamine eluent were identified, achieving 55% lysozyme activity from egg white recovery.
Area of Science:
- Biochemistry
- Biomaterials Science
- Enzyme Immobilization
Background:
- Chitosan, a natural polyaminosaccharide, is a versatile biomaterial.
- Lysozyme is a crucial enzyme with antibacterial properties.
- Enzyme immobilization enhances enzyme stability and reusability.
Purpose of the Study:
- To immobilize lysozyme onto chitosan without intermediate reagents.
- To determine optimal operating conditions for chitosan-based enzyme columns.
- To assess the activity and recovery yield of immobilized lysozyme.
Main Methods:
- Direct immobilization of lysozyme onto a chitosan matrix.
- Determination of optimal pH for column operation.
- Identification of propylamine as the most effective eluting agent.
- Enzyme activity assay using glycolchitin and Remazol Brilliant Blue R.
Main Results:
- Successful direct immobilization of lysozyme on chitosan achieved.
- Optimal pH conditions for chitosan columns were established.
- A 2% propylamine solution proved to be the best eluent.
- Lysozyme recovery from chicken egg white yielded 55% of its original activity.
Conclusions:
- Direct immobilization of lysozyme on chitosan is feasible and efficient.
- Optimized conditions enhance the performance of chitosan-based enzyme systems.
- This method offers a promising approach for lysozyme recovery and application.