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Updated: May 9, 2026

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Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues
Published on: March 9, 2021
UV-Vis as quantification tool for solubilized lignin following a single-shot steam process
Roland A Lee1, Charles Bédard, Véronique Berberi
1Industrial Research Chair on Cellulosic Ethanol, Departement of Chemical and Biotechnological Engineering, Université de Sherbrooke, Sherbrooke, Québec, Canada.
Bioresource Technology
|July 25, 2013
Summary
This study shows that UV/Vis spectroscopy can accurately quantify lignin concentrations from various biomass sources. This method is a fast and reliable analytical system for biorefineries.
Area of Science:
- Biomass valorization
- Analytical chemistry
- Spectroscopy
Background:
- Lignin quantification is crucial for biorefinery processes.
- Existing methods can be time-consuming.
- Developing rapid analytical tools is essential for process optimization.
Purpose of the Study:
- To establish UV/Vis spectroscopy as a reliable method for quantifying lignin concentration.
- To assess the suitability of UV/Vis for various biomass-derived lignins.
- To explore its potential as a Process Analytical Tool (PAT).
Main Methods:
- Lignin was extracted from diverse biomass (willow, aspen, softwood, canary grass, hemp) using steam processing.
- UV/Vis absorbance was measured at multiple wavelengths (212–287 nm).
- Correlation between absorbance and lignin concentration was analyzed.
Main Results:
- A significant correlation was found between UV/Vis absorbance and lignin concentration.
- The method proved effective for lignin from different biomass types.
- The analytical system is timesaving compared to traditional methods.
Conclusions:
- UV/Vis spectroscopy is a suitable and efficient analytical tool for lignin quantification in aqueous solutions.
- This technique can be applied across various biomass feedstocks in biorefineries.
- It holds potential for real-time process monitoring as a PAT.
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