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Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis
Published on: March 19, 2019
Pyruvate production in Candida glabrata: manipulation and optimization of physiological function
Shubo Li1,2, Xiulai Chen1,2, Liming Liu1,2
1a State Key Laboratory of Food Science and Technology and.
Critical Reviews in Biotechnology
|July 26, 2013
Summary
Candida glabrata efficiently produces pyruvate, a valuable industrial chemical. This review details methods to enhance yeast performance for cost-effective pyruvate and other chemical production.
Area of Science:
- Biotechnology
- Microbial Engineering
- Metabolic Engineering
Background:
- Candida glabrata is a yeast capable of high extracellular pyruvate accumulation from glucose.
- This characteristic enables cost-effective industrial-scale pyruvate production.
- Further optimization is needed to maximize its potential as a cell factory.
Purpose of the Study:
- To review current strategies for enhancing Candida glabrata performance for pyruvate production.
- To discuss systems biology approaches for understanding C. glabrata metabolism.
- To explore future perspectives for engineering C. glabrata for chemical synthesis.
Main Methods:
- Optimization of vitamin and dissolved oxygen levels.
- Regulation of intracellular cofactor concentrations.
- Enhancement of environmental robustness of C. glabrata.
- Application of systems biology for metabolic analysis.
Main Results:
- Current advances focus on optimizing culture conditions and cellular metabolism.
- Systems biology provides insights into C. glabrata metabolic pathways.
- Engineering efforts aim to improve pyruvate yield and broaden product range.
Conclusions:
- Candida glabrata holds significant potential for biotechnological production of pyruvate and other valuable compounds.
- Continued research in metabolic engineering and systems biology will unlock its full capabilities.
- C. glabrata can be engineered into a versatile cell factory for sustainable chemical manufacturing.
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