Related Experiment Video
Updated: May 9, 2026

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
Published on: October 31, 2019
Mutational analysis of cj0183 Campylobacter jejuni promoter
Agnieszka Salamasznska-Guz1, Marta Grodzik, Danuta Klimuszko
1Department of Pre-Clinical Sciences, Warsaw University of Life Sciences, Warsaw, Poland, asalam@tlen.pl.
Abstract:
Gene-nominated cj0183 was identified in Campylobacter jejuni NCTC 11168 and in two human isolates 81116 and 81-176. It encodes a protein which shows partial homology to TlyC of Brachyspira hyodysenteriae. The aim of this work was to determine the mechanisms of gene regulation by cloning DNA fragments lying upstream of the cj0183 gene. The β-galactosidase activity determined for the strain harboring the plasmid with the fragment upstream of cj0183 indicated the presence of a promoter in this DNA region. Mutations in cj0183 -10 region, -16 region, and -35 region resulted in changes in gene transcription.
Related Concept Videos
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
Mutations in Microorganisms
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Cis-regulatory Sequences
Cis-regulatory Sequences

