Capillary zone electrophoresis with on-line blotting for separation and detection of (32)P-postlabelled DNA adducts
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Updated: May 9, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
1Walther Straub-Institut für Pharmakologie und Toxikologie, Nussbaumstr. 26, D-80336 München, Germany.
Abstract:
A new technique for the detection of (32)P-postlabelled DNA adducts separated by capillary electrophoresis was developed. By direct transfer from the capillary outlet to a positively charged moving filter paper, eluted radioactive peaks can be quantified using a phosphor imaging detector. With this method it is possible to separate DNA adducts from different carcinogens after (32)P-postlabelling of the modified and unmodified nucleotides with high sensitivity approaching 1 adduct per 10(9) nucleotides.
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