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Updated: May 9, 2026

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
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Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

Chitosan nanoparticles: preparation, size evolution and stability.

Antonio Rampino1, Massimiliano Borgogna, Paolo Blasi

  • 1Laboratory of Physical and Macromolecular Chemistry, Dept. Life Sciences, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

International Journal of Pharmaceutics
|July 27, 2013
PubMed
Summary
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Chitosan-tripolyphosphate nanoparticles were reproducibly synthesized using ionotropic gelation. Bioprotectants like trehalose enhanced nanoparticle stability during drying and storage, confirming their safety in vivo.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Polymer Chemistry

Background:

  • Chitosan-tripolyphosphate nanoparticles are promising drug delivery vehicles.
  • Controlling nanoparticle characteristics is crucial for their efficacy.
  • Understanding nanoparticle formation and stability is essential for reproducible production.

Purpose of the Study:

  • To characterize chitosan-tripolyphosphate nanoparticles.
  • To correlate particle properties (shape, size, surface chemistry) with preparation methods.
  • To investigate the impact of chitosan molecular weight and loaded proteins on nanoparticle characteristics.

Main Methods:

  • Nanoparticles prepared via dropwise addition of tripolyphosphate to chitosan solutions.
  • Ionotropic gelation method employed for synthesis.
Keywords:
Chitosan nanoparticlesFreeze-dryingIonotropic gelationNanoparticle ageingProtein carriersSpray drying

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Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
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Last Updated: May 9, 2026

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
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Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

  • Bioprotectants (trehalose, mannitol, polyethylene-glycol) used to enhance stability during freeze-drying and spray-drying.

    • Main Results:

    • A novel bimodal particle size distribution was observed due to particle rearrangement.
    • Nanoparticle size increased slightly upon 4-week storage.
    • All tested bioprotectants improved nanoparticle stability; trehalose was most effective.
    • In vivo tests in chick embryos confirmed biocompatibility.

    Conclusions:

    • The ionotropic gelation method is effective for producing reproducible chitosan-tripolyphosphate nanoparticles.
    • Low molecular weight chitosan facilitates desired physico-chemical and safety characteristics.
    • The developed nanoparticles exhibit good biocompatibility.