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Related Experiment Video

Updated: May 9, 2026

Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
10:01

Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation

Published on: July 10, 2012

Mastering mouse lung transplantation from scratch--a track record.

Yukio Tsushima1, Jae-Hwi Jang, Moritz C Wurnig

  • 1Division of Thoracic Surgery, University Hospital Zurich, Zurich, Switzerland; Department of General Thoracic Surgery, Juntendo University School of Medicine, Tokyo, Japan.

The Journal of Surgical Research
|July 30, 2013
PubMed
Summary

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Researchers can master mouse lung transplantation without prior microsurgery experience. This study details a training protocol that significantly reduces complications and improves success rates, encouraging broader adoption of this vital research model.

Area of Science:

  • Surgical innovation
  • Transplantation research
  • Animal models

Background:

  • Mouse lung transplantation is an increasingly utilized scientific model.
  • Acquiring this technique is challenging for those without prior microsurgical experience.
  • Sharing challenges faced during model development can encourage new researchers.

Purpose of the Study:

  • To describe a training protocol for mouse lung transplantation designed for surgeons without prior microsurgical experience.
  • To document the learning curve and complication rates during the development of this technique.
  • To encourage broader adoption of mouse lung transplantation in research settings.

Main Methods:

  • Utilized inbred (C57BL/6, BALB/c, SVG129) and CD1-outbred mice as donors and recipients.
Keywords:
ComplicationsLung transplantationMouse

More Related Videos

Intravital Two-Photon Microscopy of the Transplanted Mouse Lung
04:16

Intravital Two-Photon Microscopy of the Transplanted Mouse Lung

Published on: April 19, 2024

Related Experiment Videos

Last Updated: May 9, 2026

Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
10:01

Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation

Published on: July 10, 2012

Intravital Two-Photon Microscopy of the Transplanted Mouse Lung
04:16

Intravital Two-Photon Microscopy of the Transplanted Mouse Lung

Published on: April 19, 2024

  • A surgeon inexperienced in microsurgery followed a structured training protocol: donor preparation, implantation into deceased recipients, then into surviving recipients.
  • Transplant viability was assessed using micro-computed tomography (micro-CT).

    • Main Results:

    • Donor preparation complications reduced from 43% to 0% within two months.
    • Successful implantation into surviving recipients was achieved by day 60.
    • Intraoperative complication rates, primarily anastomosis failure, decreased from 58% to 15%.
    • The final transplantation success rate reached 82% ± 2.8% with an average procedure time of 75 ± 4.8 minutes.
    • A modified forceps improved venous anastomosis completion.

    Conclusions:

    • Consistent success in mouse lung transplantation is achievable without prior microsurgical expertise.
    • The technique can be mastered within a reasonable timeframe using a limited number of training animals.
    • Minimizing complications is possible through corrective steps during critical surgical phases, encouraging researchers new to microsurgery to adopt this model.