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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...

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Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
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Supported liquid extraction as an alternative to solid phase extraction for LC-MS/MS aldosterone analysis?

Laura J Owen1, Brian G Keevil

  • 1Biochemistry Department, University Hospital of South Manchester, Manchester, UK. Laura.owen@uhsm.nhs.uk

Annals of Clinical Biochemistry
|July 31, 2013
PubMed
Summary

Supported liquid extraction (SLE) is a viable alternative to solid phase extraction (SPE) for measuring aldosterone using liquid chromatography tandem mass spectrometry (LC-MS/MS). SLE significantly reduces sample preparation time while maintaining analytical accuracy.

Keywords:
Calibrationaldosteronemass spectrometry

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Published on: September 23, 2021

Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Supported liquid extraction (SLE) is a novel sample preparation technique.
  • Compared to established methods like solid phase extraction (SPE), liquid-liquid extraction (LLE), and protein precipitation (PPE), SLE offers potential advantages.
  • This study evaluates SLE as an alternative to SPE for aldosterone measurement.

Purpose of the Study:

  • To assess the suitability of SLE as a replacement for SPE in aldosterone analysis.
  • To compare the performance of two different SLE 96-well plate devices against SPE.
  • To determine the impact of SLE on sample preparation time.

Main Methods:

  • Analysis of 83 patient samples using routine SPE.
  • Subsequent analysis of the same samples using two distinct SLE 96-well plate devices (Thermo and Biotage) with methyl-tertiary butyl ether.
  • Direct comparison of SPE and SLE techniques on two mass spectrometers.

Main Results:

  • SLE demonstrated excellent agreement with SPE results for aldosterone quantification.
  • Biotage SLE plates yielded considerably higher area counts compared to Thermo SLE plates.
  • No significant differences were observed in aldosterone measurement accuracy between SPE and SLE.

Conclusions:

  • Supported liquid extraction (SLE) is an acceptable and effective alternative to SPE for LC-MS/MS analysis of aldosterone.
  • SLE offers a significant reduction in sample preparation time.
  • SLE provides a valuable alternative for laboratories seeking to optimize aldosterone analysis workflows.