Related Experiment Video
Updated: May 9, 2026

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
Published on: March 15, 2018
[Monolithic molecularly imprinted column-high performance liquid chromatography for enrichment and determination of
Lin Sun1, Fuyou Du, Guihua Ruan
1College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.
Abstract:
A method based on monolithic molecularly imprinted polymer enrichment combining with high performance liquid chromatography (mMIP-HPLC) detection was developed for the selective determination of trace cytokinins (CTKs) in plant samples. Monolithic molecularly imprinted polymer (mMIP) column was prepared in stainless steel tube by using kinetin as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, and toluene-dodecanol as the porogenic solvents. Compared with non-imprinted polymer (NIP) monolith, the prepared mMIP exhibited selective separation ability, good reproducibility and reusability, and high extraction efficiency in the separation and enrichment of the four CTKs. Under the optimized experimental conditions, mean recoveries were 91.9%, 80.0%, 87.5% and 50.2% for kinetin (K), kinetin glucoside (KR) , trans-zeatin (tZ) and meta-topolin (mT), respectively, with the corresponding RSDs less than 11.8%. The proposed mMIP-HPLC method was successfully applied in the separation and determination of the four cytokinins in different plant samples
More Related Videos
11:44Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Published on: January 19, 2022
06:25Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Related Concept Videos
Ion-Exchange Chromatography
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation