Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Complexometric Titration: Ligands

Complexometric Titration: Ligands

Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...

EDTA: Chemistry and Properties

EDTA: Chemistry and Properties

Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Serum pro-N-cadherin: a biomarker of cardiac fibrosis and diastolic dysfunction in irradiated non-human primates.

bioRxiv : the preprint server for biology·2026

Same author

Updates on Von Willebrand Disease Testing.

Clinics in laboratory medicine·2026

Same author

Developing And Internally Validating AI-Based Aging Resilience Biomarkers in Non-Human Primates.

bioRxiv : the preprint server for biology·2026

Same author

Developing and internally validating AI-based aging resilience biomarkers in non-human primates.

The journals of gerontology. Series A, Biological sciences and medical sciences·2026

Same author

Total-body Irradiation Reduces Cerebrovascular Reactivity in Rhesus Macaques (Macaca mulatta).

International journal of radiation oncology, biology, physics·2026

Same author

γ-Radiation Induces Long-Term Dose-Related Proteomic and Phosphorylated-Tau Species Changes in Non-Human Primate Hippocampus.

Research square·2026

Same journal

Assessing Human Epidermal Growth Factor Receptor 2 in Urothelial Carcinoma: Insights From Clinical Practice Into Scoring Criteria, Histologic Subtypes, and Genomic Characteristics Across Disease Sites.

Archives of pathology & laboratory medicine·2026

Same journal

Cross-Reactivity of TPIT Antibody Clone OTI2G1 in Chordoma: Structural Mechanisms and Diagnostic Implications.

Archives of pathology & laboratory medicine·2026

Same journal

Paracoccidioidomycosis at Autopsy: A Case Series and Literature Review.

Archives of pathology & laboratory medicine·2026

Same journal

Accuracy of Cytology Diagnosis for Well Differentiated Neuroendocrine Tumors: Assessment by the College of American Pathologists Non-Gynecologic Slide Program.

Archives of pathology & laboratory medicine·2026

Same journal

Serum Immunofixation Electrophoresis Guidance Conflict: A Call to Harmonize.

Archives of pathology & laboratory medicine·2026

Same journal

In Reply.

Archives of pathology & laboratory medicine·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 9, 2026

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Published on: May 5, 2017

D-dimer: simple test, tough problems.

John D Olson¹, Mark T Cunningham, Russell A Higgins

¹Department of Pathology, University of Texas Health Science Center, San Antonio, TX 78229-3900, USA. olsonj@uthscsa.edu

Archives of Pathology & Laboratory Medicine

|August 1, 2013

Summary

This summary is machine-generated.

Laboratory practices for D-dimer testing show significant variability in methods and reporting, impacting venous thromboembolism (VTE) diagnosis. Despite interventions, inconsistencies persist, affecting test accuracy and patient care.

More Related Videos

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Published on: January 20, 2015

Related Experiment Videos

Last Updated: May 9, 2026

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Published on: May 5, 2017

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Published on: January 20, 2015

Area of Science:

Clinical Chemistry
Hematology
Diagnostic Laboratory Medicine

Background:

D-dimer assays are crucial for diagnosing venous thromboembolism (VTE).
Significant variations exist in D-dimer assay methods and laboratory applications.
Standardization is needed for reliable VTE exclusion and diagnosis.

Purpose of the Study:

To investigate current laboratory practices for D-dimer assay and reporting.
To identify sources of variability in D-dimer testing.
To assess the impact of these variations on VTE diagnosis.

Main Methods:

Analysis of D-dimer proficiency testing data.
Survey of laboratory practices for D-dimer performance and reporting.
Comparison of laboratory practices with manufacturer recommendations.

Main Results:

High variability observed in D-dimer proficiency testing across different methods.
33% of labs altered units, increasing proficiency testing failure rates.
Many labs used non-standard thresholds or qualitative assays for VTE exclusion.

Conclusions:

Considerable variability exists within and among quantitative D-dimer assay methods.
Laboratory practices in reporting units and setting VTE exclusion thresholds vary widely.
Interventions have not fully resolved the persistent variability in D-dimer testing.