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Updated: May 9, 2026
Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry
Published on: September 5, 2013
[Extraction of fatty constituents from Tabanus bivittatus and their analysis by GC-MS]
Cheng-Hua Ding1, Feng-Pu Cao, Yan-Hua Wang
1School of Chemistry and Pharmaceutical Engineering/School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China. dingdingxy2004@163.com
Objective:
To optimize the extracting condition of fatty constituents from Tabanus bivittatus and analyse by GC-MS.
Methods:
Taking fatty yield as index, the extraction technology parameters of fatty constituents of Tabanus bivittatus ere optimized by single factor test and orthogonal test.
Results:
The optimum extraction parameters were as follows: the ratio of acetone to petroleum ether 1: 2, extraction time 2 h, solid-liquid ratio 1: 50. Twenty-one fatty acids were identified by GC-MS.
Conclusion:
The process is reasonable and with good reproducibility. The main components of fatty acids in Tabanus bivittatus are palmitoleic acid, palmitic acid, linoleic acid, oleic acid and stearic acid.
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