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[HPLC fingerprint study for the quality evaluation of atractylodis macrocephalae rhizoma]
Xiang-Dong Chen1, Guang-Da Zhang, Xiao-Yong Lan
1Guangdong Yifang Pharmaceutical Co., Ltd., Foshan 528244, China. chenxd66@126.com
Objective:
To establish an HPLC fingerprint analysis method for Atractylodis Macrocephalae Rhizoma (warm dried).
Methods:
Took methanol extract of Atractylodis Macrocephalae Rhizoma as sample by ultrasonic processing, HPLC analysis was carried out on Sinochrom ODS-BP (4.6 mm x 250 mm, 5 microm) chromatographic column with mobile phase of acetonitrile (A)-water (B), gradient elution with a flow at 1.0 mL/min, ultraviolet detection wavelength at 242 nm and column temperature at 30 degrees C.
Results:
An HPLC fingerprint analysis method for Atractylodis Macrocephalae Rhizoma was developed. Eight common peaks were obtained and three peaks were identified.
Conclusion:
The method is accurate and credible which can be used for quality control of Atractylodis Macrocephalae Rhizoma (warm dried).
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