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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Production of Biopesticides01:18

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

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Related Experiment Video

Updated: May 9, 2026

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
10:14

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

RNAi for insect control: current perspective and future challenges.

Rajan Katoch1, Amit Sethi, Neelam Thakur

  • 1Biochemistry Laboratory, Department of Crop Improvement, College of Agriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, HP, 176062, India, rajankatoch@yahoo.com.

Applied Biochemistry and Biotechnology
|August 2, 2013
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) shows promise for insect pest control, with oral delivery of double-stranded RNA (dsRNA) being efficient. Challenges in dsRNA delivery and systemic RNAi machinery require further research for practical application.

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Entomology

Background:

  • RNA interference (RNAi) is a rapidly advancing field for insect pest management.
  • Genomic sequencing facilitates the application of RNAi technology across diverse insect groups.
  • Effective delivery of double-stranded RNA (dsRNA) remains a critical hurdle for practical RNAi-based pest control.

Purpose of the Study:

  • To review the prospects and challenges of using RNAi for insect control.
  • To highlight the significance of oral dsRNA delivery as an efficient application method.
  • To discuss the current understanding and remaining questions regarding systemic RNAi in insects.

Main Methods:

  • Review of existing research on RNA interference (RNAi) in insect pest control.
  • Analysis of different double-stranded RNA (dsRNA) delivery methods, focusing on oral delivery.
  • Examination of molecular mechanisms involved in dsRNA uptake and systemic spread.

Main Results:

  • RNAi-mediated gene knockdown has demonstrated significant success in various insect groups.
  • Oral delivery of dsRNA is identified as a practical and efficient approach for insect control.
  • Uptake of dsRNA involves SID-1/SID-2 proteins or endocytosis, but systemic RNAi pathways are not fully elucidated.

Conclusions:

  • RNAi technology holds substantial potential as a future strategy for insect pest management.
  • Overcoming challenges in dsRNA delivery and understanding systemic RNAi are crucial for successful implementation.
  • Further research is needed to fully realize the potential of RNAi in sustainable agriculture and public health.