Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase
Mayumi Niiyama1, Shigeru Sugiyama, Mika Hirose
1Graduate School of Science, Osaka University, Suita, Osaka 565-0871, Japan.
Abstract:
The spermidine acetyltransferase (SAT) from Escherichia coli catalyses the transfer of acetyl groups from acetyl-CoA to spermidine. SAT has been expressed and purified from E. coli. SAT was crystallized by the sitting-drop vapour-diffusion method to obtain a more detailed insight into the molecular mechanism. Preliminary X-ray diffraction studies revealed that the crystals diffracted to 2.5 Å resolution and belonged to the cubic space group P23, with unit-cell parameters a = b = c = 148.7 Å. They contained four molecules per asymmetric unit.
