Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation of Optical-Based Sensors for the Determination of Cardiac Myosin-Binding Protein C.

ACS omega·2026
Same author

Ceragenin-Loaded Tri-Layered Skin Substitute Composed of Natural and Synthetic Biopolymers for Burn Wound Healing.

Journal of biomedical materials research. Part A·2026
Same author

Molecularly imprinted polymers: Recent advances in protein chromatography.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same author

Immunoaffinity biosensors for deoxynivalenol determination from wheat sample: the evaluation of antibody immobilization route and surface topography on the sensor performance.

Mikrochimica acta·2026
Same author

Ultrasensitive Human Urinary Albumin Detection via Composite Nanohydrogels.

Micromachines·2026
Same author

Fabrication and characterization of black seed oil-loaded poly(lactic-co-glycolic acid)/pullulan nanofibers for biomedical applications.

International journal of biological macromolecules·2026

Related Experiment Video

Updated: May 9, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Microcontact imprinted surface plasmon resonance sensor for myoglobin detection.

Bilgen Osman1, Lokman Uzun, Necati Beşirli

  • 1Uludag University, Department of Chemistry, Bursa, Turkey.

Materials Science & Engineering. C, Materials for Biological Applications
|August 6, 2013
PubMed
Summary

This study developed a novel surface plasmon resonance (SPR) sensor using molecular imprinting for sensitive and selective myoglobin detection in human serum, achieving a detection limit of 87.6 ng/mL.

Keywords:
Microcontact imprintingMolecularly imprinted polymerMyoglobinNanofilmSPR sensor

More Related Videos

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Related Experiment Videos

Last Updated: May 9, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Myoglobin detection is crucial for diagnosing acute myocardial infarction.
  • Existing detection methods may lack sensitivity or specificity.
  • Molecular imprinting offers a promising approach for targeted analyte recognition.

Purpose of the Study:

  • To develop a highly sensitive and selective surface plasmon resonance (SPR) sensor for myoglobin detection.
  • To utilize the molecular imprinting technique for creating specific binding sites for myoglobin.
  • To evaluate the sensor's performance in detecting myoglobin in human serum samples.

Main Methods:

  • Synthesized myoglobin-imprinted poly(HEMA-MATrp) nanofilms on SPR sensor surfaces.
  • Characterized the nanofilms using techniques like AFM, XPS, and ellipsometry.
  • Evaluated sensor performance with varying myoglobin concentrations and human serum using SPR.

Main Results:

  • The SPR sensor demonstrated effective myoglobin detection with a detection limit of 87.6 ng/mL.
  • Langmuir adsorption model best described the sensor's binding behavior.
  • The sensor exhibited high selectivity for myoglobin over other proteins like lysozyme and BSA.

Conclusions:

  • The developed SPR sensor, utilizing molecular imprinting, is a sensitive and selective tool for myoglobin detection.
  • This sensor shows potential for clinical applications in diagnosing conditions like acute myocardial infarction.
  • The poly(HEMA-MATrp) nanofilm provides a robust platform for SPR-based biosensing.