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High-throughput micro-solid phase extraction on 96-well plate using dodecyl methacrylate-ethylen glycol
Habib Bagheri1, Ali Es'haghi, Ali Es-haghi
1Environmental and Bio-Analytical Laboratories, Department of Chemistry, Sharif University of Technology, P.O. Box 11365-9516, Tehran, Iran. bagheri@sharif.edu
Analytica Chimica Acta
|August 6, 2013
Summary
A new 96-micro-solid phase extraction (96-μ-SPE) device enables high-throughput analysis of nine pesticides in water. This method offers reliable and reproducible pesticide determination in environmental samples.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Materials Science
Background:
- Pesticide contamination in aquatic environments poses risks to ecosystems and human health.
- Accurate and efficient methods are needed for multiresidue pesticide determination in water samples.
Purpose of the Study:
- To develop and validate a novel high-throughput 96-micro-solid phase extraction (96-μ-SPE) device.
- To determine nine pesticides in aquatic samples using the developed 96-μ-SPE system coupled with gas chromatography-mass spectrometry.
Main Methods:
- Construction of a 96-μ-SPE device using stainless steel meshes and a monolithic polymer (dodecyl methacrylate and ethylene glycol dimethacrylate).
- Optimization of extraction parameters including polymer synthesis, sorbent reproducibility, ionic strength, and extraction time.
- Analysis of extracted pesticides using gas chromatography-mass spectrometry (GC-MS).
Main Results:
- The 96-μ-SPE device demonstrated high throughput and good intra- and inter-sorbent reproducibility.
- Limits of detection were below 4μg L⁻¹, with coefficients of determination (r²) greater than 0.99 for all analytes.
- The method was successfully applied to the extraction and determination of pesticides in real surface water samples.
Conclusions:
- The developed 96-μ-SPE device is a robust and efficient tool for the multiresidue determination of pesticides in aquatic samples.
- This high-throughput method provides a reliable approach for environmental monitoring of pesticide contamination.
