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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

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Related Experiment Video

Updated: May 9, 2026

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
08:39

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Published on: December 12, 2025

Probing plasma membrane dynamics at the single-molecule level.

Xiaojuan Li1, Doan-Trung Luu, Christophe Maurel

  • 1College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China; Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

Trends in Plant Science
|August 6, 2013
PubMed
Summary
This summary is machine-generated.

Single-molecule imaging reveals plant plasma membrane dynamics. Advanced microscopy techniques like TIRFM, FCS, and super-resolution microscopy offer new insights into membrane organization and protein diffusion in living plant cells.

Keywords:
membrane dynamicsmembrane microdomainssingle molecule

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Last Updated: May 9, 2026

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
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Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
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Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
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Area of Science:

  • Plant cell biology
  • Biophysics
  • Microscopy

Background:

  • Plant plasma membranes are dynamic and respond to environmental cues.
  • Conventional microscopy limits understanding of membrane dynamics due to low spatial resolution.

Purpose of the Study:

  • To review single-molecule imaging methods for studying plant plasma membranes.
  • To highlight advances in understanding membrane organization and protein dynamics.

Main Methods:

  • Total Internal Reflection Fluorescence Microscopy (TIRFM)
  • Fluorescence Correlation Spectroscopy (FCS)
  • Super-resolution microscopy

Main Results:

  • Single-molecule imaging provides high spatial resolution for plant cell membranes.
  • These methods reveal details of membrane microdomain organization.
  • Insights into the diffusion dynamics of single molecules within the membrane.

Conclusions:

  • Single-molecule imaging is crucial for understanding plant plasma membrane properties.
  • Advanced microscopy techniques have significantly improved our knowledge of membrane organization and protein dynamics in plants.