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Nitidine chloride inhibits renal cancer cell metastasis via suppressing AKT signaling pathway

Zhiqing Fang1, Yueqing Tang, Wei Jiao

  • 1Department of Urology, Qilu Hospital of Shandong University, Ji'nan, Shandong, China; The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital of Shandong University, Ji'nan, Shandong, China.

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
|August 6, 2013
PubMed

Insights

Nitidine Chloride (NC) effectively inhibits renal cancer cell migration and invasion. This compound shows potential as an anti-metastasis agent by impacting AKT signaling pathways.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Nitidine Chloride (NC) exhibits anti-cancer properties in various tumor types.
  • The anti-metastasis potential and mechanisms of NC in renal cancer remain largely unexplored.

Purpose of the Study:

  • To investigate the anti-metastasis effects of Nitidine Chloride (NC) on renal cancer cells.
  • To elucidate the underlying molecular mechanisms of NC's action in renal cancer metastasis.

Main Methods:

  • In vitro studies utilizing scratch and Transwell assays to assess cell migration and invasion.
  • Analysis of AKT phosphorylation and matrix metalloproteinase (MMP-2 and MMP-9) expression levels.
  • Evaluation of combined NC and AKT inhibitor (LY294002) effects.

Main Results:

  • NC significantly suppressed the migration and invasion of 786-O and A498 renal cancer cell lines.
  • NC treatment led to decreased AKT phosphorylation and reduced levels of MMP-2 and MMP-9.
  • Co-administration of NC with an AKT inhibitor potentiated the anti-metastasis effects.

Conclusions:

  • Nitidine Chloride demonstrates significant in vitro anti-metastasis activity against renal cancer cells.
  • NC exerts its effects partly through the inhibition of the AKT signaling pathway.
  • NC presents a promising therapeutic candidate for developing anti-metastasis strategies in renal cancer.
Keywords:
AKTAKT/PKBDMEMDMSODimethylsulfoxideDulbecco’s Modified Eagle’s MediumFBSFetal Bovine SerumMMPMetastasisNitidine chloridePBSPBSTPhosphate Buffered SalinePhosphate Buffered Saline and Tween 20RAC-alpha serine/threonine-protein kinaseRenal cancermatrix metalloproteinase

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