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Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method
Published on: October 23, 2013
EDTA enhanced phytoremediation of copper contaminated soils using chickpea (Cicer aeritinum L.)
Murty S Kambhampati1, Van Tu Vu
1Department of Natural Sciences, Southern University at New Orleans, 6400 Press Dr., New Orleans, LA 70126, USA. mkambham@suno.edu
Abstract:
The goal of this research was to determine whether or not chickpea (Cicer arietinum L.), commonly known as Garbanzo beans, is a hyper accumulator for copper (Cu) in contaminated soils amended with EDTA. Statistical analysis (2 tailed Pearson Correlation) revealed significant correlations between: Translocation index and stem biomass (r = 0.859**; p < 0.01); Tolerance index and stem biomass (r = 0.762**; p < 0.01); and bioconcentration factor of stem/soil and soil Cu concentration (r = -0.545*; p < 0.05). Therefore, C. arietinum seems to be a cost-effective and environmentally friendly hyperaccumulator for Cu at 100 ppm Cu and 10 mM EDTA.
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In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...