Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 9, 2026

Isolation of Embryonic Ventricular Endothelial Cells

Isolation of Embryonic Ventricular Endothelial Cells

Published on: July 20, 2013

Isolation of embryonic ventricular endothelial cells.

Laura A Dyer¹, Cam Patterson

¹McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Journal of Visualized Experiments : Jove

|August 6, 2013

Summary

Researchers developed a new method to isolate primary embryonic endothelial cells for studying coronary vasculature development. This technique enables detailed analysis of developing blood vessels in transgenic mouse models.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

IL-28A/IL-10Rβ axis promotes angiogenesis via eNOS/AKT signaling and AP-1/NF-κB/MMP-2 network by regulating HSP70-1 expression.

Journal of advanced research·2024

Same author

Design and Impact of a Novel Rural Hospital Alliance.

Southern medical journal·2024

Same author

CRAT links cholesterol metabolism to innate immune responses in the heart.

Nature metabolism·2023

Same author

RESPONSE: Last Fair Deal Gone Down.

Journal of the American College of Cardiology·2022

Same author

Atrogin-1 inhibits Akt-dependent cardiac hypertrophy in mice via ubiquitin-dependent coactivation of Forkhead proteins.

The Journal of clinical investigation·2022

Same author

Fetal Blood Flow and Genetic Mutations in Conotruncal Congenital Heart Disease.

Journal of cardiovascular development and disease·2021

Area of Science:

Cardiovascular Biology
Developmental Biology
Cell Biology

Background:

Cell culture is vital for studying cell populations.
Immortalized cell lines are not available for all cell types.
Primary cells from transgenic mice allow for more detailed studies.

Purpose of the Study:

To adapt a cell isolation technique for embryonic organs.
To study developing coronary vasculature in transgenic mouse embryos.
To enable analysis of specific cell populations during development.

Main Methods:

Dissection of embryonic mouse organs and dissociation into single cells.
Isolation of specific cell types using antibody-conjugated magnetic beads.
Modification of the technique for small embryonic samples and low cell yields.

More Related Videos

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Published on: January 23, 2014

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Published on: June 17, 2016

Related Experiment Videos

Last Updated: May 9, 2026

Isolation of Embryonic Ventricular Endothelial Cells

Isolation of Embryonic Ventricular Endothelial Cells

Published on: July 20, 2013

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Published on: January 23, 2014

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Published on: June 17, 2016

Main Results:

Successful isolation of transgenic embryonic ventricular endothelial cells.
Assessment of coronary plexus remodeling in these cells.
Demonstration of a scaled-down protocol for embryonic cell isolation.

Conclusions:

The modified technique is effective for isolating embryonic endothelial cells.
This method facilitates the study of coronary vasculature development.
Primary cell isolation from transgenic embryos offers valuable research insights.