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Updated: Jun 17, 2025
07:49
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
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IL-28A/IL-10Rβ axis promotes angiogenesis via eNOS/AKT signaling and AP-1/NF-κB/MMP-2 network by regulating HSP70-1
Jun-Hui Song1, Byungdoo Hwang1, Sung Lyea Park1
1Department of Food and Nutrition, Chung-Ang University, Anseong 456-756, Korea.
Journal of Advanced Research
|August 10, 2024
Summary
Interleukin-28A (IL-28A) promotes angiogenesis by activating heat shock protein 70-1 (HSP70-1) and the IL-10 receptor beta (IL-10Rβ) pathway. This study reveals IL-28A
Area of Science:
- Molecular Biology
- Cell Biology
- Physiology
Background:
- Angiogenesis is crucial for tumor progression and inflammatory diseases.
- The role and regulatory mechanisms of IL-28A in angiogenesis are not well understood.
Purpose of the Study:
- To investigate the novel regulatory role of IL-28A in physiological angiogenesis.
- To elucidate IL-28A-mediated angiogenic mechanisms and identify key genes involved.
Main Methods:
- Human umbilical vein endothelial cells (HUVECs) were used to assess proliferation, migration, invasion, and tube formation.
- Next-generation sequencing (NGS) analyzed gene expression changes.
- Animal models (aortic ring, Matrigel plug, hind-limb ischemia) and siRNA knockdown were employed to evaluate functional roles.
Main Results:
- IL-28A enhanced HUVEC proliferation, migration, invasion, and tube formation via eNOS/AKT and ERK1/2 signaling.
- HSP70-1 was identified as a key effector gene, mediating IL-28A-induced angiogenesis.
- IL-10Rβ is essential for IL-28A-stimulated angiogenic responses.
Conclusions:
- HSP70-1 mediates angiogenesis through the IL-28A/IL-10Rβ axis.
- The pathway involves eNOS/AKT signaling and the AP-1/NF-κB/MMP-2 network.
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