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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: May 9, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Northern analysis of gene expression.

Lita A Freeman1

  • 1Cardiovascular & Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 6, 2013
PubMed
Summary

Northern analysis is a technique to detect and quantify RNA transcripts. This method provides insights into gene expression, transcript size, and abundance, avoiding PCR artifacts.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Gene expression analysis is crucial for understanding cellular function.
  • Accurate detection and quantification of RNA transcripts are essential for molecular biology research.

Purpose of the Study:

  • To describe the Northern analysis technique for RNA transcript detection and quantification.
  • To highlight the utility of Northern analysis in studying gene expression patterns.

Main Methods:

  • RNA extraction from biological samples.
  • RNA denaturation and size-based electrophoresis.
  • Blotting RNA onto a membrane followed by hybridization with a labeled probe.
  • Washing to remove non-specific binding and detection of specific transcripts.

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Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

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Related Experiment Videos

Last Updated: May 9, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
05:22

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

Published on: July 29, 2022

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Main Results:

  • Northern analysis enables the detection and quantification of full-length RNA transcripts.
  • The method provides information on transcript size, abundance, and alternative splicing.
  • It can confirm the presence or absence of specific exons within transcripts.

Conclusions:

  • Northern analysis is a valuable tool for studying gene expression, offering insights into transcript variants and abundance.
  • The technique avoids Polymerase Chain Reaction (PCR) amplification artifacts.
  • It provides a comprehensive view of gene transcripts and novel insights into gene expression.