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Purification of plasmid and BAC transgenic DNA constructs

Chengyu Liu1, Yubin Du, Wen Xie

  • 1iPSC and Genome Engineering Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 6, 2013
PubMed
Summary

High-quality DNA is essential for successful transgenic mouse generation via pronuclear microinjection. This guide details protocols to ensure DNA purity, concentration, and integrity, crucial for embryo development and experimental success.

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