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Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
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The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits
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Published on: August 20, 2014

Surface modifications by polymers for biomolecule conjugation.

Laura Sola1, Marina Cretich, Francesco Damin

  • 1Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare, Milan, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|August 7, 2013
PubMed
Summary
This summary is machine-generated.

Polymeric coatings offer enhanced binding capacity for biomolecular recognition. This study details "grafting to" and "grafting from" methods for functionalizing glass and silicon surfaces with custom copolymers.

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Area of Science:

  • Materials Science and Engineering
  • Surface Chemistry
  • Biotechnology

Background:

  • Polymeric coatings provide homogenous surface derivatization with high reactive group concentration, increasing target binding capacity.
  • These coatings act as linkers, distributing bound probes axially for faster biomolecular recognition reactions.
  • Polymer attachment to surfaces is achieved via "grafting to" or "grafting from" approaches.

Purpose of the Study:

  • To present protocols for functionalizing glass and silicon surfaces using both "grafting to" and "grafting from" methods.
  • To demonstrate the use of specific copolymers for surface modification.
  • To highlight the versatility of polymeric coatings in tailoring surface properties for specific applications.

Main Methods:

  • Utilized "grafting to" and "grafting from" polymerization techniques.
  • Employed copolymers based on N,N-dimethylacrylamide (DMA) or Glycidyl methacrylate (GMA) as the polymer backbone.
  • Incorporated N-acryloyloxysuccinimide (NAS) as the reactive group and 3-(trimethoxysilyl)propyl methacrylate (MAPS) or 3-mercaptopropyl trimethoxy silane (MPS) as anchoring groups.

Main Results:

  • Successfully functionalized glass and silicon surfaces using the described protocols.
  • Demonstrated the effectiveness of the chosen copolymers and anchoring groups for surface modification.
  • Achieved homogenous surface derivatization with high reactive group concentration.

Conclusions:

  • Established protocols for surface functionalization using "grafting to" and "grafting from" approaches with specific copolymers.
  • Highlighted the potential of these tailored polymeric coatings for applications in biomolecular recognition and surface-based assays.
  • Emphasized the advantages of polymeric coatings in enhancing binding capacity and reaction kinetics.