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Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...

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Updated: May 9, 2026

Breeding by Design for Functional Rice with Genome Editing Technologies

Breeding by Design for Functional Rice with Genome Editing Technologies

Published on: January 3, 2025

Reverse genetics in rice using Tos17.

Delphine Mieulet¹, Anne Diévart, Gaëtan Droc

¹CIRAD, UMR AGAP, Montpellier cedex 5, France.

Methods in Molecular Biology (Clifton, N.J.)

|August 7, 2013

Summary

This summary is machine-generated.

Tos17 (Oryza sativa transposon 17) is an active retroelement in rice used for mutagenesis. New protocols using PCR and DNA blot analysis help identify Tos17 insertions in mutant rice lines for gene function studies.

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Area of Science:

Plant genetics
Molecular biology
Genomics

Background:

Tos17 (Oryza sativa transposon 17) is an active Ty1-Copia Class I retroelement in rice.
Tos17 is inactive under normal conditions but activates during tissue culture, leading to new genomic insertions.

Purpose of the Study:

To provide two protocols for identifying Tos17 insertions in rice.
To facilitate gene function studies using Tos17-induced mutations.

Main Methods:

PCR-based protocol to identify azygous, heterozygous, and homozygous plants.
DNA blot analysis to confirm homozygous insertions and clean mutant backgrounds.

Main Results:

Tos17 copies transpose and insert into new genomic locations after tissue culture.
Established protocols enable precise identification of Tos17 insertions in mutant rice populations.

Conclusions:

The developed protocols are crucial for researchers utilizing Tos17 insertion mutants.
These methods support both forward and reverse genetics approaches for deciphering rice gene function.