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Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
LTR Retrotransposons03:08

LTR Retrotransposons

LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Updated: May 9, 2026

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
11:36

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Published on: September 23, 2017

Transposon display: a versatile method for transposon tagging.

Michiel Vandenbussche1, Jan Zethof, Tom Gerats

  • 1UMR 5667 CNRS-INRA-ENS Lyon-Unversité Lyon I, RDP Laboratory, ENS Lyon, Lyon, Cedex, France.

Methods in Molecular Biology (Clifton, N.J.)
|August 7, 2013
PubMed
Summary
This summary is machine-generated.

Transposon Display, an updated technique, enables robust visualization of transposon-flanking sequences for cloning mutants. This method aids in analyzing transposon behavior and forward cloning, especially in species with high transposable element copy numbers.

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Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

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Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • Transposon tagging is a key method for cloning mutants in various organisms.
  • High copy numbers and transposition rates of elements pose challenges for forward cloning.
  • Existing methods may lack the resolution needed for complex genomes.

Purpose of the Study:

  • To present an updated Transposon Display technique for visualizing numerous transposon-flanking sequences.
  • To enable robust and reproducible forward cloning of mutants in challenging species.
  • To analyze the transpositional behavior of the dTph1 transposon in Petunia.

Main Methods:

  • Adaptation of the Amplified Fragment Length Polymorphism (AFLP) technique.
  • Simultaneous visualization of a large number of transposon-flanking sequences.
  • Application in the Petunia W138 background with high dTph1 element copy numbers.

Main Results:

  • The updated Transposon Display technique provides a highly robust and reproducible visualization method.
  • Successful application for forward cloning of mutants in Petunia.
  • Demonstrated ability to handle species with over 200 copies of endogenous transposable elements.

Conclusions:

  • The enhanced Transposon Display technique is effective for forward cloning and transposon behavior analysis.
  • The method is adaptable to different biological systems and transposable elements.
  • Offers a high-resolution solution for mutant cloning in complex genomes.