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Different polarisome components play distinct roles in Slt2p-regulated cortical ER inheritance in Saccharomyces

Xia Li1, Susan Ferro-Novick, Peter Novick

  • 1Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093-0644 Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093-0644.

Molecular Biology of the Cell
|August 9, 2013
PubMed

Insights

The protein phosphatase Ptc1p is crucial for endoplasmic reticulum (ER) inheritance in yeast. Its absence traps ER on the cytoskeleton, linked to the Slt2p kinase activity at the bud tip.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cortical endoplasmic reticulum (cER) inheritance is essential for cell division.
  • Ptc1p, a type 2C protein phosphatase, plays a role in cER inheritance in Saccharomyces cerevisiae.
  • Defects in Ptc1p lead to ER tubules failing to spread around the bud cortex.

Purpose of the Study:

  • To investigate the role of the polarisome complex in cER inheritance.
  • To elucidate the mechanism by which Ptc1p regulates cER inheritance via Slt2p.
  • To understand the connection between ER inheritance, Slt2p activity, and the actin cytoskeleton.

Main Methods:

  • Genetic analysis of yeast mutants (ptc1Δ, polarisome components, Sec3p).
  • Observation of ER tubule dynamics and localization.
  • Assessment of Slt2p (cell wall integrity mitogen-activated protein kinase) activation and retention.
  • Investigation of actin cytoskeleton involvement through depolymerization.

Main Results:

  • The polarisome complex components Spa2p and Pea2p are required for Slt2p retention at the bud tip.
  • Other polarisome components (Bni1p, Bud6p, Sph1p) influence Slt2p activation levels.
  • Actin depolymerization rescues the cER inheritance defect in ptc1Δ cells, indicating ER is trapped on the cytoskeleton.
  • Loss of Sec3p also blocks ER inheritance, activates Slt2p, and is rescued by actin depolymerization.

Conclusions:

  • Slt2p activity at the bud tip is a key regulator of ER inheritance.
  • ER inheritance is linked to the association of the ER with the actin-based cytoskeleton.
  • A common mechanism involving Slt2p and the cytoskeleton regulates ER inheritance in yeast.

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