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Updated: May 9, 2026
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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Detecting polymorphisms in human longevity studies: HLA typing and SNP genotyping by amplicon sequencing
Gilberto Vargas-Alarcón1, Carmina Flores-Domínguez
1Department of Molecular Biology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, Mexico.
Methods in Molecular Biology (Clifton, N.J.)
|August 10, 2013
Summary
Genetics significantly influence human lifespan, with common single-nucleotide polymorphisms (SNPs) playing a key role in longevity. Understanding genetic variations, like Human Leukocyte Antigen (HLA) allele polymorphism, is crucial for aging research.
Area of Science:
- Genetics and Aging Research
- Molecular Biology
- Immunology
Background:
- Human lifespan is influenced by both environmental and genetic factors, with genetics accounting for 25-32% of individual differences in longevity.
- Aging involves cellular changes, and longevity is the capacity to manage and repair this damage, often linked to immune system function.
- Longevity gene candidates encompass immune-related factors, stress responses, metabolism, DNA repair, and cellular proliferation.
Purpose of the Study:
- To explore the genetic underpinnings of human longevity and aging.
- To detail methodologies for detecting genetic polymorphisms associated with lifespan.
- To highlight the role of immune and inflammatory gene variants in longevity.
Main Methods:
- Sequencing of Class I Human Leukocyte Antigen (HLA) allele polymorphism.
- Single-Nucleotide Polymorphism (SNP) sequencing techniques.
- Analysis of polygenic effects influencing lifespan.
Main Results:
- Common Single-Nucleotide Polymorphisms (SNPs) are significantly related to human lifespan.
- Polygenic effects contribute substantially to the genetic influence on longevity.
- Class I HLA allele polymorphism and SNP sequencing are key methods for detecting relevant genetic variations.
Conclusions:
- Genetic polymorphisms, particularly SNPs and HLA variations, are critical determinants of human longevity.
- Advanced sequencing methodologies are essential for identifying genetic factors contributing to aging and lifespan.
- Further research into these genetic elements can enhance our understanding of aging processes.
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