The potential for PCR based testing to improve diagnosis and treatment of sepsis

Early and appropriate antibiotic use is critical for the treatment of sepsis. In practice, however, early antibiotic administration is hampered by diagnostic uncertainty with regard to both early diagnosis of disease and delayed or absent microbiological results. The diagnostic uncertainty often persists into the later stages of sepsis when clinical response to antibiotic treatment is assessed. Blood culture, the current "gold standard" test for diagnosis of sepsis, is inadequate due to low sensitivity and delayed results. However, despite this, the technology remains entrenched in microbiology laboratories due to decades of accumulated experience with the technique. However, in recent years there has been increasing experience with testing based on polymerase chain reaction (PCR). As the technology continues to improve, PCR testing is becoming faster and more sensitive. Moreover, PCR technologies that quantify bacterial load may enable monitoring of response to treatment and susceptibility testing.