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Updated: May 9, 2026

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
10:22

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices

Published on: September 2, 2009

A Microfluidic Passive Pumping Coulter Counter.

Amy L McPherson1, Glenn M Walker

  • 1Department of Biomedical Engineering, North Carolina State University, Raleigh & University of North Carolina at Chapel Hill, NC, Tel.: 919-513-8253 almcpher@ncsu.edu.

Microfluidics and Nanofluidics
|August 10, 2013
PubMed
Summary
This summary is machine-generated.

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This study demonstrates a passive pumping microfluidic device for particle counting. The device offers comparable accuracy to traditional methods, making it a viable alternative for particle enumeration.

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Particle Analysis

Background:

  • Microfluidic devices offer miniaturized platforms for various applications.
  • Accurate particle counting is crucial in diagnostics and research.
  • Traditional pumping methods can be complex and expensive.

Purpose of the Study:

  • To characterize a microfluidic device utilizing passive pumping for particle counting.
  • To evaluate the accuracy of passive pumping against syringe pumping and hemocytometry.
  • To assess the impact of washing steps on particle count accuracy.

Main Methods:

  • A microfluidic device with passive pumping was designed and fabricated.
  • Polystyrene particles (10 µm) were counted using the resistive pulse technique.
Keywords:
ColloidEnumerationMicrofluidicsResistive Pulse

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Last Updated: May 9, 2026

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
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Published on: September 2, 2009

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
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  • Particle counts were compared between passive pumping, syringe pumping, and hemocytometry.
  • The effect of varying wash droplet volumes on particle counts was investigated.

    • Main Results:

    • Passive pumping particle counts were within 13% of syringe pumping.
    • All pumping methods showed results within 16% of hemocytometer counts.
    • No significant difference in particle counts was observed with varying wash steps (p > 0.05).
    • Hydrodynamic focusing was successfully demonstrated with passive pumping.

    Conclusions:

    • Passive pumping is a viable and accurate method for particle counting in microfluidic devices.
    • The device's performance is robust, unaffected by intermediate wash steps.
    • This technology presents a simpler and potentially more cost-effective alternative for particle analysis.