Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 9, 2026

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells
05:49

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

Published on: April 13, 2018

Robust, efficient, and practical electrogene transfer method for human mesenchymal stem cells using square electric

Aaron Liew1, Franck M André, Léa L Lesueur

  • 11 Regenerative Medicine Institute, National Centre for Biomedical Engineering Science, National University of Ireland Galway , Galway, Ireland .

Human Gene Therapy Methods
|August 13, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Method for Adapting Euglena gracilis to Cassava-Based Substrates for Heterotrophic Cultivation.

Journal of visualized experiments : JoVE·2026
Same author

Electrical stimulation and stem cell subdural implantation decrease microglia reactivity after spinal cord injury.

Materials today. Bio·2026
Same author

How One Single Eureka Moment Can Dictate a Full Scientific Career in Bioelectricity.

Bioelectricity·2026
Same author

Label-free DNA-sensor modelling based on magnetic induction spectroscopy.

BioTechniques·2026
Same author

The effect of GLP-1 receptor agonists on cognition in nondiabetic patients with mild cognitive impairment or alzheimer's disease: a meta-analysis of randomized controlled trials.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2026
Same author

The biomedical engineer's pledge: overview and context.

Medical & biological engineering & computing·2025

This study optimized electrogene transfer for human mesenchymal stem cells (hMSCs), achieving over 80% transfection efficiency while maintaining cell viability and differentiation potential for regenerative medicine applications.

Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Biotechnology

Background:

  • Mesenchymal stem cells (MSCs) are crucial for regenerative medicine due to their differentiation capacity.
  • Electrogene transfer is a safe method for genetically modifying MSCs.
  • Previous studies achieved 32% transfection efficiency in rat MSCs.

Purpose of the Study:

  • To enhance transfection efficiency and transgene expression in human MSCs (hMSCs) using electrogene transfer.
  • To preserve hMSC viability and differentiation potential post-transfection.
  • To establish a robust and reproducible protocol for genetically engineered hMSCs for clinical use.

Main Methods:

  • Optimized electrogene transfer parameters including plasmid concentration and buffer osmotic pressure.

More Related Videos

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
08:11

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Published on: January 31, 2019

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential
07:41

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Published on: January 18, 2019

Related Experiment Videos

Last Updated: May 9, 2026

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells
05:49

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

Published on: April 13, 2018

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
08:11

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Published on: January 31, 2019

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential
07:41

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Published on: January 18, 2019

  • Utilized a square-wave electric pulse generator for transfection.
  • Assessed transfection efficiency, cell viability, and differentiation into osteoblasts and adipocytes.

    • Main Results:

    • Achieved over 80% transfection efficiency in hMSCs with approximately 70% cell viability.
    • Demonstrated sustained transgene expression for up to 30 days.
    • Confirmed reproducibility across bone marrow and adipose-derived hMSCs, with no significant donor or confluency effects. Cryopreservation maintained cell function.

    Conclusions:

    • Developed a robust, safe, and highly efficient electrogene transfer protocol for hMSCs.
    • The optimized protocol preserves hMSC viability and multipotency, suitable for clinical applications.
    • Provides practical insights for utilizing genetically engineered hMSCs in regenerative medicine.