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Mild hypothermia inhibits differentiation of human embryonic and induced pluripotent stem cells

Glenn S Belinsky1, Srdjan D Antic

  • 1Department of Neuroscience, University of Connecticut Health Center, Farmington, CT, USA. belinsky@uchc.edu

Biotechniques
|August 13, 2013
PubMed

Culture of pluripotent stem cells at 35°C strikingly reduces unwanted spontaneous differentiation during hESC and iPSC maintenance compared with 37°C. Growth at 35°C did not affect expression of pluripotency mRNAs nor induce expression of cold-inducible genes. Colony size was somewhat reduced at 35°C. Thus, growth at 35°C is a convenient, simple method to reduce the labor of removing spontaneously differentiated colonies when maintaining pluripotent cells.

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