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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: May 9, 2026

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
10:44

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Published on: August 15, 2025

Future-generation sequencing and clinical microbiology.

Benjamin C Kirkup1, Steven Mahlen, George Kallstrom

  • 1Department of Wound Infections, Walter Reed Army Institute of Research, Room 3A24, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.

Clinics in Laboratory Medicine
|August 13, 2013
PubMed
Summary

Next-generation sequencing is transforming laboratory medicine and clinical practice. Challenges remain in data analysis and interpretation for clinical microbiology labs integrating these powerful nucleic acid sequencing tools.

Keywords:
Clinical microbiologyGenomicsSequencing

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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Related Experiment Videos

Last Updated: May 9, 2026

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
10:44

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Published on: August 15, 2025

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Area of Science:

  • Clinical microbiology
  • Laboratory medicine
  • Genomic technologies

Background:

  • Sequencing technologies are increasingly impacting patient care.
  • The clinical microbiology laboratory is a key area for the application of sequencing.
  • Massive data sets from sequencing are becoming integrated into clinical practice.

Purpose of the Study:

  • To review the benefits and limitations of future sequencing technologies in clinical microbiology.
  • To highlight the challenges in data analysis and interpretation.
  • To discuss the integration of sequencing into clinical practice.

Main Methods:

  • Review of current and emerging sequencing technologies.
  • Analysis of data handling and interpretation infrastructure.
  • Discussion of educational and analytical needs for clinical laboratories.

Main Results:

  • Sequencing offers significant potential benefits for clinical microbiology.
  • Current infrastructure struggles to keep pace with sequencing advancements.
  • Effective interpretation of massive sequencing data sets is a major limitation.

Conclusions:

  • Sequencing technologies promise to revolutionize clinical microbiology.
  • Addressing infrastructure and data interpretation challenges is crucial for successful implementation.
  • Education and training are essential for leveraging sequencing in patient care.