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Related Experiment Video

Updated: May 9, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Next generation sequencing for human papillomavirus genotyping.

L Sara Arroyo¹, Vitaly Smelov, Davit Bzhalava

¹Department of Laboratory Medicine, Karolinska Institutet and Karolinska University Hospital, 141 86 Stockholm, Sweden.

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology

|August 13, 2013

Summary

Next-generation sequencing (NGS) offers a sensitive and accurate method for human papillomavirus (HPV) genotyping. This approach aids in tracking HPV variants and monitoring vaccination impact.

Keywords:

CIN GS GS junior HPV High throughput sequencing Human papillomavirus Luminex MID NGS Next generation sequencing cervical intraepithelial neoplasia emPCR emulsion PCR genome sequencer human papillomavirus multiplex identifier next generation sequencing

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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

Area of Science:

Virology
Genetics
Molecular Biology

Background:

Human papillomavirus (HPV) genotyping is crucial for epidemiological studies.
Monitoring HPV variants is important following HPV vaccination programs.
Next-generation sequencing (NGS) offers potential for HPV variant analysis.

Purpose of the Study:

To design and validate a rapid method for HPV detection, typing, and sequencing.
To assess the utility of NGS for clinical HPV sample analysis.

Main Methods:

Developed an NGS-based method using PGMY primers and 454 sequencing.
Tested serially diluted HPV plasmids (1-100 copies/sample).
Compared NGS results with Luminex-based genotyping in 60 cervical samples and assessed reproducibility in 33 samples.

Main Results:

NGS correctly identified 15 HPV types at 100 copies/sample and 13/15 at 10 copies/sample.
Identical genotyping results between NGS and Luminex in 36/60 samples.
NGS demonstrated higher sensitivity than Luminex in 12/60 samples, with good reproducibility.

Conclusions:

NGS is a sensitive and accurate method for HPV genotyping.
Obtaining amplicon sequences via NGS is valuable for studying viral variants and monitoring HPV vaccination effectiveness.