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Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
Published on: February 18, 2020
An improved protocol for electroporation in members of the genus Gordonia
Pooja Singh1, Preeti Srivastava
1Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Journal of Microbiological Methods
|August 13, 2013
Abstract:
Gordonia are high GC gram-positive bacteria that have not yet been exploited well for biotechnological purposes because of the limited genetic tools. Described here is an improved protocol for electroporation, which is useful for several Gordonia species. The maximum transformation efficiency obtained was 2.8 × 10(4)/μg (Gordonia rubropertinctus, Gordonia sp), and 1.7 × 10(3)/μg (Gordonia amarae).
