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Updated: May 8, 2026

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
Published on: December 3, 2011
Environmental surveillance of human parechoviruses in sewage in The Netherlands
W J Lodder1, M Wuite, A M de Roda Husman
1Laboratory for Zoonoses and Environmental Microbiology at the Centre for Infectious Disease Control Netherlands, National Institute of Public Health and the Environment (RIVM).
Abstract:
The circulation of human parechoviruses (HPeVs) in the population was studied by environmental surveillance comprising of molecular analyses of sewage samples (n = 89) that were collected from 15 different locations in The Netherlands. Samples were taken from sewage originating from schools (n = 9) or from parts of municipalities (n = 6) during the Dutch school year 2010-2011. At 13/15 locations HPeV1, HPeV3, or HPeV6 RNA was detected at least once; however, sequence diversity did not reflect associations in time or place. A higher percentage of positives was observed in the samples originating from the municipalities. It was demonstrated that HPeV circulated in the studied population to a higher extent than would be expected from the current knowledge on infections predominating in young children.

